![]() ![]() DIA fragments all masses within a predefined set of m/ z ranges, usually spanning the range of useful peptide masses from approximately 400 to 1000 m/ z. ![]() In contrast, DIA scans are the same in each sample analysis. (4−6) As the name implies, the scan sequence in DDA depends on the data even if the same sample is analyzed twice, the scans collected in each analysis are unique. Two main types of MS data acquisition exist, namely, data-dependent analysis (DDA) (1−3) and data-independent analysis (DIA). Shotgun proteomics using liquid chromatography (LC) coupled to tandem mass spectrometry (MS/MS) is currently the leading method to identify and quantify proteome dynamics from biological samples.
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